Coding

Part:BBa_K3008001:Design

Designed by: Shayan Vahdani   Group: iGEM19_UCSC   (2019-09-24)


Group 3 LEA 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 805
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Induced a silent mutation by changing GCT to GCG (alanine) to remove PvuII site (position 165). The sequence was codon optimized for E. coli using a self-written program from the Silver lab at Harvard.

Source

This gene is from the genome of Artemia franciscana.

References